Elevated salivary C-reactive protein levels are associated with active and passive smoking in healthy youth: A pilot study
© Azar and Richard; licensee BioMed Central Ltd. 2011
Received: 7 July 2011
Accepted: 7 December 2011
Published: 7 December 2011
We examined salivary C-reactive protein (CRP) levels in the context of tobacco smoke exposure (TSE) in healthy youth. We hypothesized that there would be a dose-response relationship between TSE status and salivary CRP levels.
This work is a pilot study (N = 45) for a larger investigation in which we aim to validate salivary CRP against serum CRP, the gold standard measurement of low-grade inflammation. Participants were healthy youth with no self-reported periodontal disease, no objectively measured obesity/adiposity, and no clinical depression, based on the Beck Depression Inventory (BDI-II). We assessed tobacco smoking and confirmed smoking status (non-smoking, passive smoking, and active smoking) with salivary cotinine measurement. We measured salivary CRP by the ELISA method. We controlled for several potential confounders.
We found evidence for the existence of a dose-response relationship between the TSE status and salivary CRP levels.
Our preliminary findings indicate that salivary CRP seems to have a similar relation to TSE as its widely used serum (systemic inflammatory) biomarker counterpart.
KeywordsSalivary C-reactive protein active and passive tobacco smoke exposure cotinine
The province of New Brunswick (NB), where this pilot study took place, has one of the highest smoking rates in Canada at 18.1% (with 19.9% of males and 16.5% of females smoking daily) . Undergraduate years are a time of stress and exploration. Consequently, many youth develop permanent smoking habits, and about one-third of them will become addicted to cigarette smoking . Both active and passive tobacco smoke exposure (TSE) are related to an increased risk of cardiovascular events and diseases [3, 4], by altering the autonomic function or by inducing pro-inflammatory responses [4–6].
Inflammation, acute and systemic, is part of our immune reaction and leads to the release of C-reactive protein (CRP) into the bloodstream [7–9]. However, chronically increased serum CRP is a risk factor for the development of coronary artery disease (CAD) . For example, chronic pro-inflammatory responses play an important role in the induction and progression of atherosclerosis . Understanding the mechanisms through which TSE may affect CRP levels in young smokers is central to the prevention of early atherosclerosis. This is fundamental as CAD is one of the leading causes of death of Canadian adults. Furthermore, heart disease and stroke cost the Canadian economy more than $22.2 billion each year .
Serum CRP, the gold standard measurement of low-grade inflammation, can predict future cardiac events, even in healthy individuals . Thus, serum CRP is often utilized to assess future CAD risk. However, serum testing is invasive in nature. In contrast, the salivary CRP assay, commercially available since November 2008 (e.g., Salimetrics Europe), is minimally invasive and yet apparently sufficiently sensitive [12, 13], compared to serum CRP. It is therefore ideal for use in research on TSE in youth.
The aim of this pilot study was to investigate salivary CRP levels in the context of TSE in healthy youth. Based on findings with serum CRP , we tested the hypothesis of a dose-response relationship between TSE status (active, passive, non-smoking) and salivary CRP levels. Specifically, we hypothesized that salivary CRP levels would rise in a dose-response manner with the extent of TSE.
Study design & participants
Exclusion Criteria for Participants
There is a positive relationship between salivary CRP and periodontal disease, which could help to explain the underlying link between periodontal disease and higher risk for CAD .
(based on body mass index measurement).
Obesity is a major determinant of elevated serum CRP .
Apparent clinical levels of depressive symptoms
There is increasing evidence of a relationship between depressive symptoms and elevated serum CRP, putting individuals at increased cardiac risk . Depression has also been shown to be related to TSE .
We used the Tobacco Use Questionnaire (TUQ), adapted from O'Loughlin et al. , which exhibited good reliability and validity with a Canadian youth population. The items were selected from a set of questions pertaining to smoking history that were used in the Québec Child and Adolescent Health Social Survey (QCAHSS); the latter were extracted from the 1994 Canadian Survey of Smoking among youths . The TUQ asks about rate of smoking, type of tobacco products used, and if participants are/were exposed to tobacco smoke in their past or current living environments. We used the TUQ to identify non-smokers, passive smokers, and smokers (≥ 1 cigarette/day, on a daily basis). We confirmed TSE status with salivary cotinine measurement (Salimetrics, State College, PA, USA). The intra-assay coefficient of variation (CV) of the cotinine assay was 6.5%. The inter-assay CV was 4.21% for low values and 9.04% for high values. Cotinine high sensitivity enzyme immunoassays (EIA) were performed in duplicate. The repeated EIAs were highly correlated (r = .99, p < 0.001). Smokers had significantly higher cotinine levels (M = 97.34, SD = 138.80) than passive smokers (M = 0.69, SD = 0.96), t(12) = 3.15, p = 0.009. Smokers also had higher cotinine levels than non-smokers (M = .83, SD = 1.11), t(12) = 3.15, p = 0.009. However, cotinine levels were not significantly different between passive smokers and non-smokers (p > 0.05).
Salivary CRP levels
Salivary CRP is the main and single outcome measure of this study. Although salivary CRP does not appear to be flow rate dependent , flow saliva rate was determined while collecting the samples. In order to avoid any possible fluctuations in CRP levels, we tested female participants between the 4th and 10th day of the menstrual cycle. The saliva samples were immediately stored at -20°C until analysis (to avoid bacterial growth and loss of CRP). The concentrations of salivary CRP levels were measured by the ELISA method (Salimetrics, LLC, Pennsylvania, USA). For optimal quality-control, the same technician analyzed all samples. The % of cross-reactivity was < 0.004 for several compounds, including human albumin, IL-6, lysozyme, and alpha 1-Antitrypsin. The intra-assay CV of the CRP assay was 1.9% and the inter-assay CV was 3.7%. CRP assays were run in duplicates. Again, the repeated measures were highly correlated, r > .99, p < .001.
Potential confounders & statistical analyses
Based on the available literature on systemic inflammation [8, 15, 16], we specified a priori the following potential covariates: cold/flu or infection within the past 2 weeks, systemic diseases such as diabetes mellitus, medication use such as statins, aspirin, contraceptive pills, age, body mass index (measured in kg/m2), non-clinical levels of depressive symptoms (BDI-II), and other substance use. The substance use was determined from the Simple Screening Instrument for Substance Abuse (SSI-SA), which is appropriate for use with youth . Similarly, we established the following a priori potential covariates for cotinine measurement: intake of tea, tomatoes, potatoes and eggplants as these contain small amounts of nicotine that may contribute to the salivary cotinine levels . To test our hypothesis, we compared differences in salivary CRP levels of non-smoking, passive smoking, and active smoking groups using an ANOVA and post-hoc t-tests.
This pilot study explored the immunomodulatory effects of TSE among healthy youth, as indexed by salivary CRP. CRP levels were highest in active smokers, lower in passive smokers, and lowest in non-smokers. This supported our hypothesis of a "dose-response" effect of TSE on salivary CRP levels. This finding corroborated results of studies using the more widely used serum CRP measure [5, 9, 14, 19]. Thus our preliminary findings suggests that salivary CRP seems to have a similar relation to TSE as its widely used serum (systemic inflammatory) biomarker counterpart .
A recent study  investigated salivary and serum CRP in healthy medical students. Although not focusing on smoking, high correlations between the two CRP biomarkers were not found. However, the study was limited by: 1. the absence of age-related reference ranges for the salivary CRP test and 2. a lack of control for periodontal disease. The latter is significant as elevated salivary CRP levels have been shown to indicate poor periodontal health or chronic oral infections (related to tissues/structures supporting the teeth). In addition, oral chronic infections have been linked to CAD . More precisely, this link might be partly explained by systemic chronic inflammation [4, 21], possibly in the form of subtle persistent low-grade inflammation originating from oral chronic infections.
Our study is among the very scarce literature on CRP in human saliva. To the best of our knowledge, this is the first investigation examining salivary CRP levels as a function of TSE levels. The strengths of this pilot study include taking into account several potential confounders. Most importantly, we ruled out the effects of reported periodontal disease on the findings (exclusion criterion). Ideally, assessing plaque induced gingivitis, while recording more objective indices of periodontal health, would have been a more precise measure of oral health. Although we have used a high-sensitivity salivary EIA assay, it is possible that this assay was not sensitive enough to capture any difference in cotinine levels between passive smokers and non-smokers. Furthermore, self-reports of undesirable social behaviours, like smoking, may be prone to under-reporting in some populations (e.g., ill older adults, pregnant women). Although under-reporting is insignificant in healthy youth, potential misclassification of smokers due to self-reporting remains a possibility. This limitation is mitigated by the use of cotinine testing. Cotinine, which is a specific, sensitive valid biomarker for determining exposure to "tobacco/nicotine", confirmed the integrity of the self-reported smoking data. Finally, different levels of physical activity, between participants in the TSE groups (active, passive), might have influenced our results. As the pilot testing did not measure physical activity, this remains a possibility that deserves future investigation .
Inflammation may be an important physiological mechanism by which tobacco use increases risk for atherosclerosis and eventually CAD . Thus, a practical use of our finding of dose-response relationship between TSE status and salivary CRP in young healthy smokers would be to: 1) Provide more evidence to the importance of the inflammatory pathway underlying cardiovascular risky behaviours in healthy youth and 2) Perhaps more importantly, suggest promising evidence that a simple, less invasive, & non-painful biomarker might be an alternative to the more invasive serum measure of CRP.
Our pilot finding is encouraging as it has the potential to support the future application of salivary CRP assays in populational research, and possibly eventually in clinical settings (at least in the context of young populations exposed to tobacco smoke). Further research should examine the relationship between changes in salivary CRP with serum CRP and investigate the stability of the salivary CRP biomarker over extended periods of time. These are the next steps to our pilot study. Once these steps are completed, we could assess whether the salivary CRP test has the potential to be a non-invasive and practical clinical tool to monitor risk for cardiovascular diseases in large populations.
Dr. Rima Azar is the recipient of a CIHR/RPP New Investigator Award. This research was supported by two grants: # 48-1-595949 from Marjorie Young Bell Start-up Award and # 31-1-505095 from Marjorie Young Bell Faculty Award. We are grateful to participants for their time. We would like to thank Ms. Julie Lewis for proofreading the final version of this manuscript.
- Statistics Canada: 2009, CANSIM, table 105-0427 and Catalogue no. 82-221-X, 2009Google Scholar
- DiFranza JR, Savageau JA, Fletcher K, O'Loughlin J, Pbert L, Ockene JK, McNeill AD, Hazelton J, Friedman K, Dussault G, Wood C, Wellman R: Symptoms of tobacco dependence after brief intermittent use: The Development and Assessment of Nicotine Dependence in youth - 2 study. Arch Pediatr Adolesc Med. 2007, 161: 704-710. 10.1001/archpedi.161.7.704.PubMedView ArticleGoogle Scholar
- Ridker PM: High-sensitivity C-reactive protein, inflammation, and cardiovascular risk: From concept to clinical practice to clinical benefit. Am Heart J. 2004, 148: 19-26. 10.1016/j.ahj.2004.04.028.View ArticleGoogle Scholar
- Willershausen B, Kasaj A, Zahorka D, Briseño B, Blettner M, Genth-Zotz D, Münzel T: Association between chronic dental infection and acute myocardial infarction. J Endodontics. 2009, 35: 626-630. 10.1016/j.joen.2009.01.012.View ArticleGoogle Scholar
- Wannamethee SG, Lowe GDO, Shaper AG, Rumley A, Lennon L, Whincup PH: Associations between cigarette smoking, pipe/cigar smoking, and smoking cessation, and haemostatic and inflammatory markers for cardiovascular disease. Eur Heart J. 2005, 26: 1765-1773. 10.1093/eurheartj/ehi183.PubMedView ArticleGoogle Scholar
- Hastie CE, Haw S, Pell JP: Impact of smoking cessation and lifetime exposure on C-reactive protein. Nic Tob Res. 2008, 10: 637-642. 10.1080/14622200801978722.View ArticleGoogle Scholar
- Nordestgaard BG, Zacho J: Lipids, atherosclerosis and CVD risk: Is CRP an innocent bystander?. Nutr Metab Cardiovasc Dis. 2009, 19: 521-524. 10.1016/j.numecd.2009.07.005.PubMedView ArticleGoogle Scholar
- Azar R, Nolan R, Stewart D: Listening to the heart-brain talk: persistent depressive symptoms are associated with hsCRP in apparently healthy individuals at high risk for coronary artery disease". Eur J Cardiovasc Prev Rehabil. published online before print, July 5, 2011Google Scholar
- Ohsawa M, Okayama A, Nakamura M, Onado T: CRP levels are elevated in smokers but unrelated to the number of cigarettes and are decreased by long-term smoking cessation in make smokers. Prev Med. 2005, 41: 651-656. 10.1016/j.ypmed.2005.02.002.PubMedView ArticleGoogle Scholar
- Canadian Heart Health Strategy and Action Plan (CHHS-AP): 2009, OttawaGoogle Scholar
- Clearfield MB: C-Reactive Protein: A new risk assessment tool for cardiovascular disease. J Am Osteopath. Assoc. 2005, 105 (9): 409-416.PubMedGoogle Scholar
- Ouellet-Morin I, Danese A, Williams B, Arsenault L: Validation of a high-sensitivity assay for C-reactive protein in human saliva. Brain Beh Imm. 2011, 25: 640-646. 10.1016/j.bbi.2010.12.020.View ArticleGoogle Scholar
- Salimetrics: Salivary C-reactive protein ELISA kit. 2009, Salimetrics cat # 1-3302, 1-3302-5Google Scholar
- O'Loughlin J, Lambert M, Karper I, MacGrath J: Association between cigarette smoking and C-reactive protein in a representative, population-based sample of adolescents. Nic Tob Res. 2008, 10 (3): 525-532. 10.1080/14622200801901997.View ArticleGoogle Scholar
- Noack B, Genco RJ, Grossi S, Zambon JJ, De Nardin E: Periodontal infections contribute to elevated systemic C-reactive protein level. J Periodont. 2001, 72: 1221-1227. 10.1902/jop.2000.72.9.1221.PubMedView ArticleGoogle Scholar
- Panagiotakos DB, Pitsavos C, Chrysohoou C, Tsetsekou E, Papageorgiou C, Christodoulou G, Stefanadis C: Inflammation, coagulation, and depressive symptomatology in cardiovascular disease-free people; the ATTICA study. Eur Heart J. 2004, 25: 492-499. 10.1016/j.ehj.2004.01.018.PubMedView ArticleGoogle Scholar
- Knight JR, Goodman E, Pulerwitz T, DuRant RH: Reliabilities of short substance abuse screening tests among adolescent medical patients. Pediatr. 2000, 105: 948-953.Google Scholar
- Siegmund B, Leitner E, Pfannhauser W: Determination of the nicotine content of various edible nightshades (solanaceae) and their products and estimation of the associated dietary nicotine intake. J Agric Food Chem. 1999, 47: 3113-3120. 10.1021/jf990089w.PubMedView ArticleGoogle Scholar
- Fröhlich M, Sund M, Löwel H, Imhof A, Hoffmeister A, Koenig W: Independent association of various smoking characteristics with markers of systemic inflammation in men: Results from a representative sample of the general population (MONICA Augsburg survey 1994/95. Eur Heart J. 2003, 24: 1365-1372. 10.1016/S0195-668X(03)00260-4.PubMedView ArticleGoogle Scholar
- Janeway CA, Travers P, Walport M, Shlomchik MJ: Immunobiology: The Immune System in Health and Disease. 2005, New York: Garland Publishing, 6Google Scholar
- Dillon MC, Opris DC, Lickliter J, Cornwell HN, Bridges EG, Nazar AM, Bridges KG: Detection of Homocysteine and C-Reactive Protein in the Saliva of Healthy Adults: Comparison with Blood Levels. Biomark Insights. 2010, 5: 57-61.PubMedPubMed CentralGoogle Scholar
- Tremblay MS, Shephard RJ, McKenzie TL, Gledhill N: Physical activity assessment options within the context of the Canadian Physical Activity, Fitness, and Lifestyle Appraisal. Can J App Physiol. 2001, 26 (4): 388-407. 10.1139/h01-024.View ArticleGoogle Scholar
- He J, Vupputuri S, Whelton PK: Relationship between cigarette smoking and novel risk factors for cardiovascular disease in the United States. Ann Intern Med. 2003, 138: 891-897.PubMedView ArticleGoogle Scholar
- Beck AT, Steer RA, Garbin MG: Psychometric properties of the Beck Depression Inventory: twenty-five years of evaluation. Clin Psychol Rev. 1988, 8: 77-100. 10.1016/0272-7358(88)90050-5.View ArticleGoogle Scholar
- Bouteyre E, Maurel M, Bernaud JL: Daily hassles and depressive symptoms among first year psychology students in France: the role of coping and social support. Stress Health. 2007, 23 (2): 93-99. 10.1002/smi.1125.View ArticleGoogle Scholar
- Lipps GE, Lowe GA, Young R: Validation of the beck depression inventory-II in a Jamaican university student cohort. West Ind Med J. 2007, 56 (5): 404-8.Google Scholar
- Slade GD, Offenbacher S, Beck JD, Heiss G, Pankow JS: Acute-phase inflammatory response to periodontal disease in the US Population. J Dent Res. 2000, 79: 49-57. 10.1177/00220345000790010701.PubMedView ArticleGoogle Scholar
- Wee CC, Mukamal KJ, Huang A, Davis RB, McCarthy EP, Mittleman MA: Obesity and C-reactive protein levels among White, Black, and Hispanic US adults. Obesity. 2008, 16: 875-880. 10.1038/oby.2008.7.PubMedPubMed CentralView ArticleGoogle Scholar
- Cardiovascular Risk in Young Finns Study, Elovainio M, Keltikangas-Järvinen L, Pulkki-Råback L, Kivimäki M, Puttonen S, Viikari L, Räsänen L, Mansikkaniemi K, Viikari J, Raitakari OT: Depressive symptoms and C-reactive protein: The cardiovascular risk in Young Finns Study. Psychol Med. 2006, 36: 797-805. 10.1017/S0033291706007574.View ArticleGoogle Scholar
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