Histology of adipose tissue inflammation in Dercum's disease, obesity and normal weight controls: a case control study
© Hansson et al; licensee BioMed Central Ltd. 2011
Received: 8 April 2011
Accepted: 28 September 2011
Published: 28 September 2011
Dercum's disease (DD) is characterised by obesity and chronic pain (> 3 months) in the adipose tissue. The pathogenesis of DD is unknown, but inflammatory components have been proposed. In previous reports and studies, an inconsistent picture of the histological appearance of the adipose tissue in DD has been described. The aim of this investigation was to examine the histological appearance of adipose tissue in patients with DD, with particular focus on inflammatory signs.
Fat biopsies were sampled from painful regions from 53 patients with DD. In 28 of the patients, a control adipose tissue biopsy was taken from a location where the patient did not experience any pain. In addition, fat biopsies were sampled from 41 healthy pain-free obese control patients and 11 healthy pain-free normal weight control patients. The extent of inflammation was evaluated on histological sections stained with haematoxylin-eosin.
There was no statistically significant difference in the extent of inflammation between the biopsies from the painful knee and the biopsies from the non-painful area (p = 0.5), nor between the biopsies from the abdomen, and the biopsies from the non-painful area (p = 0.4), in patients with DD. A statistically significant difference in extent of inflammation was observed between DD and obese control patients regarding the abdomen (p = 0.022), but not the knee (p = 0.33). There were no differences in extent of inflammation between DD patients and normal weight controls (p = 0.81).
The findings suggest that there is an inflammatory response in the adipose tissue in DD. However, this response is not more pronounced than that in healthy obese controls. This contradicts inflammation as the aetiology of DD.
Dercum's disease (DD) is characterised by pronounced pain in the adipose tissue and a number of associated symptoms. The pain is chronic (for more than 3 months), symmetrical, often disabling  and resistant to analgesics . The pathogenesis of DD is unknown, but inflammatory components have been proposed [2–4]. However, laboratory markers for inflammation, such as erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP), are usually normal in the condition [4–17]. However, a few studies have revealed that some of the patients have elevated levels of CRP and ESR. A study from 1937 of 112 women with DD, reported that 66% had an ESR > 15 mm . Moreover, in a study by Herbst and Asare-Bediako , 33.4% of the patients with DD had elevated CRP levels and 37.5% elevated ESR levels. However, 38.2% of the patients included in the study had autoimmune disease, such as rheumatoid arthritis and lupus. In the same study 31.2% of the patients had positive titres for antinuclear antibodies (ANA). It is unclear if these patients were among the 38.2% that had an autoimmune disease. Case reports have shown that markers for autoimmune disease, such as rheumatoid factor (RF), antinuclear antibodies (ANA), anticardiolipin antibodies (ACA), perinuclear anti-neutrophil cytoplasmic antibodies (pANCA), cytoplasmic anti-neutrophil cytoplasmic antibodies (cANCA) and antibodies against native DNA, are commonly negative in DD [4, 6, 11, 15]. Regarding blood cytokines, a small study including 10 subjects and 5 controls  indicated that macrophage inflammatory protein (MIP)-1β might be lower in patients with DD than in normal controls. Moreover, a trend towards higher levels of interleukin (IL)-13 and levels of fractalkine were detected.
In previous reports and studies, an inconsistent picture of the histological appearance of the adipose tissue in DD has been described. Fat biopsies in different case reports have revealed histologically normal adipose tissue without inflammation [8, 10]. However, pathological findings have been described in other studies. Dercum originally considered the most interesting histological finding to be interstitial inflammation of the nerves in the adipose tissue of the painful sites [1, 20], which has only been confirmed in one case report .
As regards inflammatory signs in the adipose tissue in DD, leukocytes and plasma cells have been detected in two cases [3, 22]. In addition, Herbst et al.  found multi-nucleated giant (MNG) cells in three of the DD patients (n = 5) and in none of the controls (n = 5). Multi-nucleated giant cells are produced by activated, pro-inflammatory macrophages. However, no differences in number of macrophages could be seen between the patients and the controls.
Other pathological findings in fat biopsies described in DD are increased levels of connective tissue [19, 23], fibrolipoma with numerous embryonic vessels, , reactive infiltration of fibrotic elements and small angiomas , granulomas  and capillary microthrombi .
The aim of this investigation was to examine the histological appearance of the adipose tissue in patients with DD, with particular regard to inflammatory signs in a larger series of patients, and compare them with healthy, obese, body mass index (BMI)-matched controls and controls with normal BMI.
Patients and Methods
Patient profile (median and range).
Dercum (n = 53)
Obese controls (n = 41)
Normal weight controls (n = 11)
BMI (kg/m 2 )
Fat biopsies, obtained by surgical biopsy, were sampled from 53 women with DD. Biopsies were taken from painful subcutaneous fat from the abdomen and the knee region. In 28 cases, a control adipose tissue biopsy was taken from a location where the patient did not experience any pain, 21 from non-painful abdomen and 7 from non-painful knee. Fat biopsies were also sampled from the 41 obese control patients' abdomen and knee region and from the 11 normal weight control patients' knee region. All of the biopsies were open surgical biopsies taken in the same way, measuring about 15 × 15 mm.
The erythrocyte sedimentation rate (ESR) was measured using the Westergren method, that is 4 parts blood were diluted with 1 part isotonic citric solution. The level of sedimentation was measured after 1 hour. The reference intervals for ESR for Swedish women are < 21 mm up to 50 years of age, and < 30 mm between 51 and 70 years of age .
The study was approved by the Ethics of Human Investigation Committee at Lund University (LU 236-89, LU 422-91). All participants gave their written informed consent to participate. The procedures were in accordance with the Helsinki Declaration of 1964.
Values are given as medians and ranges. Histograms were drawn to examine the distribution of the measured factors. The histograms indicated that the measured factors were not normally distributed. Differences in highest inflammatory reaction between biopsies from painful locations and non-painful locations from DD patients were analysed using paired McNemar's test. Biopsies taken from the painful knee region in DD patients were compared to those from the knee region of control patients, using chi-square tests. The same procedure was used for the abdomen. In all cases the highest score for each biopsy was used when comparisons were made. Differences in ESR between the DD patients and the obese controls were analysed using the Mann Whitney test.
Inflammatory reaction (score 0 to III) in fat biopsies and intra- and intergroup differences.
Painful abdomen (n = 41)
Painful knee (n = 47)
Non-painful area (n = 28)
Abdomen (n = 41)
Knee (n = 41)
Normal weight controls
Knee (n = 11)
Differences between groups (p-value)
Dercum vs. Obese controls - abdomen
Dercum vs. Obese controls - knee
Dercum vs. Normal weight controls - knee
Obese vs. Normal weight controls - knee
Dercum - painful abdomen vs. Dercum - non-painful abdomen1
Dercum - painful knee vs. Dercum - non-painful knee2
Furthermore, no differences in extent of inflammation were detected between DD and obese control patients regarding the biopsies from the knee region (p = 0.33, χ2 = 22.2, df = 20). A significant difference in extent of inflammation was observed between DD patients and obese controls patients for the biopsies from the abdomen (p = 0.022, χ2 = 29.3, df = 16).
There were no differences in extent of inflammation between DD patients and normal weight controls (p = 0.81, χ2 = 6.1, df = 10) (knee). However, a difference was detected between obese controls and normal weight controls (p < 0.001, χ2 = 31.4, df = 8) (knee). The differences within and between the groups are summarised in Table 2.
Among the DD patients of 50 years and younger, 26 patients had an ESR of < 21 mm and 2 patients > 21 mm (24 and 38 respectively) (median 11 mm (range, 4-38)). Values were missing from three patients in the younger age group. Among the DD patients over 50 years of age, all but one had an ESR of < 30 mm (median 9.5 (range 4-34)). The patient with a higher value had an ESR of 34 mm. Values were missing from two patients in the older age group. Among the obese control patients of 50 years and younger, all but two had an ESR of < 21 mm, and two patients had values > 21 mm (29 and 90 respectively) (median 11.5 (range 2-90)). Values were missing from 5 patients in the younger age group. Among the obese control patients over 50 years of age, all but two patients had an ESR of > 30 mm (median 15 (range, 1-41)). Values were missing from 8 patients in the older age group. There was no statistical difference in ESR between the DD patients and the obese controls, neither in the age group < 50 years (p = 0.99), nor in the age group > 50 years (p = 0.73).
The strengths of the study are that the same consultant diagnosed DD in all patients and that a control group of healthy obese controls was included. Furthermore, no study has been published with a greater number of patients with DD examined through fat biopsies.
Studies in anatomical pathology as gold standard has been challenged because of the difficulties in reproducibility of histological diagnosis due to inter-observer variation. This can be explained by the fact that interpretive judgement and personal experience have to be used by the pathologist to be able to make a histopathological diagnosis . However, in the present study, the same pathologist judged all the fat biopsies in a blinded fashion, and hence, such factors should be of less influence. A limitation of the present study is that we had no information on the use of over-the-counter analgesics. It is possible that such drugs could have affected the inflammatory reaction in the adipose tissue.
An inconsistent picture of the histological appearance of the adipose tissue in DD has been reported in previous reports and studies. Recently, Herbst et al.  found multinucleated giant cells in three patients with this condition. However, when these patients were compared with healthy obese controls, no differences in the inflammatory reaction were seen. In this study, a difference in the inflammatory reaction in the adipose tissue could be seen between patients with DD and healthy obese controls comparing biopsies from the abdomen but not from the knee. In recent years, research has suggested that the adipose tissue in obesity elicits a chronic low-grade inflammatory response that contributes to co-morbidities such as diabetes, increased cardiovascular risk and liver disease [29–31]. The expanded pool of adipocytes is responsible for the increased production and release of inflammatory mediators such as cytokines. An increased density of macrophages has been observed in the adipose tissue of obese subjects [31, 32]. This can explain why DD patients and weight-matched healthy obese controls both have an elevated inflammatory reaction and the presence of macrophages in the adipose tissue.
In conclusion, our findings reveal that there is an inflammatory response in the adipose tissue in DD. However, this response might not be more pronounced than that in healthy obese controls. This contradicts inflammation as the aetiology of Dercum's disease.
The work was supported by grants from the Swedish Rheumatism Association, the insurance company Förenade Liv, Clinical Research and Development at Malmö University Hospital, Helge Wulff's Trust and the Faculty of Medicine at Lund University. We thank associate professor Birger Fagher (deceased on 21 April 2011), MD, PhD, for kindly letting us conduct research on patients in his care. We thank cytotechnologists Lars Övergaard and Thomas Lindén for help with the photos and secretary Anette Johansson for administrative help. We are indebted to Associate Professor Jonas Manjer, MD, PhD, for statistical advice.
- Dercum FX: A subcutaneous connective tissue dystrophy of the arms and back, associated with symptoms resembling myxoedema. University Medical Magazine (Philadelphia). 1888, 1: 1-11.Google Scholar
- Brorson H, Fagher B: Dercum's disease. Fatty tissue rheumatism caused by immune defense reaction?. Läkartidningen. 1996, 93: 1430, 1433-1436.Google Scholar
- Kirpila J, Ripatti N: Adiposis dolorosa juxta-articularis: Dercum's disease & its therapy. Nord Med. 1958, 59: 358-360.PubMedGoogle Scholar
- Szypula I, Kotulska A, Szopa M, Pieczyrak R, Kucharz E: Adiposis dolorosa with hypercholesterolemia and premature severe generalized atherosclerosis. Wiad Lek. 2009, 62: 64-65.PubMedGoogle Scholar
- Kosseifi S, Anaya E, Dronovalli G, Leicht S: Dercum's Disease: An Unusual Presentation. Pain Med. 2010, 11: 1430-1434. 10.1111/j.1526-4637.2010.00828.x.PubMedView ArticleGoogle Scholar
- Lange U, Oelzner P, Uhlemann C: Dercum's disease (Lipomatosis dolorosa): successful therapy with pregabalin and manual lymphatic drainage and a current overview. Rheumatol Int. 2008, 29: 17-22. 10.1007/s00296-008-0635-3.PubMedView ArticleGoogle Scholar
- Herbst KL, Asare-Bediako S: Adiposis dolorosa is more than painful fat. The Endocrinologist. 2007, 17: 326-334. 10.1097/TEN.0b013e31815942294.View ArticleGoogle Scholar
- Campen RB, Sang CN, Duncan LM: Case records of the Massachusetts General Hospital. Case 25-2006. A 41-year-old woman with painful subcutaneous nodules. N Engl J Med. 2006, 355: 714-722. 10.1056/NEJMcpc069018.PubMedView ArticleGoogle Scholar
- Tiesmeier J, Warnecke H, Schuppert F: An uncommon cause of recurrent abdominal pain in a 63-year-old obese woman. Dtsch Med Wochenschr. 2006, 131: 434-437. 10.1055/s-2006-932538.PubMedView ArticleGoogle Scholar
- Amine B, Leguilchard F, Benhamou CL: Dercum's disease (adiposis dolorosa): a new case-report. Joint Bone Spine. 2004, 71: 147-149. 10.1016/S1297-319X(03)00139-8.PubMedView ArticleGoogle Scholar
- Steiner J, Schiltz K, Heidenreich F, Weissenborn K: Lipomatosis dolorosa--a frequently overlooked disease picture. Nervenarzt. 2002, 73: 183-187. 10.1007/s00115-001-1240-9.PubMedView ArticleGoogle Scholar
- Greenbaum SS, Varga J: Corticosteroid-induced juxta-articular adiposis dolorosa. Arch Dermatol. 1991, 127: 231-233. 10.1001/archderm.127.2.231.PubMedView ArticleGoogle Scholar
- Bonatus TJ, Alexander AH: Dercum's disease (adiposis dolorosa). A case report and review of the literature. Clin Orthop Relat Res. 1986, 251-253.Google Scholar
- Nahir AM, Schapira D, Scharf Y: Juxta-articular adiposis dolorosa--a neglected disease. Isr J Med Sci. 1983, 19: 858-859.PubMedGoogle Scholar
- Eisman J, Swezey RL: Juxta-articular adiposis dolorosa: what is it? Report of 2 cases. Ann Rheum Dis. 1979, 38: 479-482. 10.1136/ard.38.5.479.PubMedPubMed CentralView ArticleGoogle Scholar
- Blomstrand R, Juhlin L, Nordenstam H, Ohlsson R, Werner B, Engstrom J: Adiposis dolorosa associated with defects of lipid metabolism. Acta Derm Venereol. 1971, 51: 243-250.PubMedGoogle Scholar
- Margherita G: Considerations on a Case of Post-Traumatic Adiposis Dolorosa Associated with a Pathologic Fracture. Rass Neuropsichiatr. 1964, 18: 211-218.PubMedGoogle Scholar
- Kling D: Juxta-articular adiposis dolorosa. Its significance and relation to Dercum's disease and osteo-arthritis. Arch Surg. 1937, 599-630.Google Scholar
- Herbst KL, Coviello AD, Chang A, Boyle DL: Lipomatosis-associated inflammation and excess collagen may contribute to lower relative resting energy expenditure in women with adiposis dolorosa. Int J Obes (Lond). 2009, 33: 1031-1038. 10.1038/ijo.2009.119.View ArticleGoogle Scholar
- Dercum FX: Two cases of adiposis dolorosa: One in a man complicated by epilepsy; another in a woman presenting also circinate retinitis. The Philadelphia medical journal. 1902, 396-399.Google Scholar
- Burr CW: A case of adiposis dolorosa, with necropsy. J Nerv Ment Dis. 1900, XXVII: 519-525.View ArticleGoogle Scholar
- Hovesen E: Adiposis doloros (Dercum's syndrome). Nord Med. 1953, 50: 971-973.PubMedGoogle Scholar
- Myers B: Case of Adiposis Dolorosa. Proc R Soc Med. 1923, 16: 11-12.PubMedPubMed CentralGoogle Scholar
- Labbé M, Boulin R: Lipomatose douloureuse et maladie de Dercum. Bull Mem Soc Med Hop Paris. 1927, 687-695.Google Scholar
- Eyckmans R: Dercum's adiposis dolorosa. Arch Belg Dermatol Syphiligr. 1954, 10: 365-366.PubMedGoogle Scholar
- Lemont H, Picciotti J, Pruzansky J: Dercum's disease. J Am Podiatry Assoc. 1979, 69: 389-391.PubMedView ArticleGoogle Scholar
- Nilsson-Ehle P, Ganrot PO, Laurell C-B: Laurells Klinisk kemi i praktisk medicin. 2003, Lund: Studentlitteratur, 8Google Scholar
- Crawford JM: Original research in pathology: judgment, or evidence-based medicine?. Lab Invest. 2007, 87: 104-114. 10.1038/labinvest.3700511.PubMedView ArticleGoogle Scholar
- Ikeoka D, Mader JK, Pieber TR: Adipose tissue, inflammation and cardiovascular disease. Rev Assoc Med Bras. 2010, 56: 116-121. 10.1590/S0104-42302010000100026.PubMedView ArticleGoogle Scholar
- Torres-Leal FL, Fonseca-Alaniz MH, Rogero MM, Tirapegui J: The role of inflamed adipose tissue in the insulin resistance. Cell Biochem Funct. 2010, 28: 623-631. 10.1002/cbf.1706.PubMedView ArticleGoogle Scholar
- Vachharajani V, Granger DN: Adipose tissue: a motor for the inflammation associated with obesity. IUBMB Life. 2009, 61: 424-430. 10.1002/iub.169.PubMedPubMed CentralView ArticleGoogle Scholar
- Ferrante AW: Obesity-induced inflammation: a metabolic dialogue in the language of inflammation. J Intern Med. 2007, 262: 408-414. 10.1111/j.1365-2796.2007.01852.x.PubMedView ArticleGoogle Scholar
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