Volume 10 Supplement 1

2nd Cross Company Respiratory Symposium

Open Access

Antiviral effect of eosinophils in respiratory tract infection with influenza virus in mice

  • Suzanne M Bal1, 2Email author,
  • Annemiek Dijkhuis1, 2,
  • Koen van der Sluijs3 and
  • Rene Lutter1, 2
Journal of Inflammation201310(Suppl 1):P24

https://doi.org/10.1186/1476-9255-10-S1-P24

Published: 14 August 2013

Rationale

Previous studies in an acute house dust mite murine model of allergic asthma have shown that allergic mice have an improved antiviral response to infection with influenza (A/PR/8/34) compared to non-allergic mice as reflected by lower viral titres in the lung and decreased weight loss. These findings demonstrate that allergic mice are able to clear the infection faster. Interestingly, we observed a synergistically enhanced eosinophilic response in bronchoalveolar lavage (BAL) of these mice in response to the viral infection.

Hypothesis

This led us to hypothesise that eosinophils are important for the antiviral response and we tested this hypothesis in IL-5 transgenic (IL-5 Tg) mice, which present with chronic eosinophilia.

Methods

IL-5 Tg mice and wild type C57BL/6 were infected with 10 TCID50 influenza (A/PR/8/34). Plasma and BAL were collected on day 4, 6 or 8 after infection and BAL cells were analysed by flow cytometry. The viral load was determined in lung tissue by qPCR. Before infection, IL-5 Tg mice had plasma levels of 500 pg/ml IL-5 and elevated eosinophils in the circulation (40% of cells), the spleen (35%), the lungs (25%) and BAL (4%).

Results

Upon influenza infection there was a significantly elevated influx of eosinophils into the lungs as measured in the BAL which peaked at day 4 after infection: IL-5 Tg animals had 6.9 * 104 eosinophils (10%) compared to 0.6 * 104 (2%) in wild type animals (p = 0.01). Equal numbers of neutrophils, macrophages and lymphocytes were found in both groups. In concurrence with our hypothesis the IL-5 Tg mice rapidly recovered from the infection. Their maximal weight loss was limited to 9%, compared to 22% in wild type animals and was paralleled by a significantly lower viral load in the Tg animals at day 8 after infection: log viral copies of 4.2 compared to 6.5 (p = 0.02).

Conclusion

These data imply that eosinophils play a crucial role in the immune response against influenza virus in mice and underline the importance of the innate antiviral immunity. Whether this applies to humans and, if so, whether this is different for patients with asthma, remains to be addressed.

Declarations

Acknowledgements

This study is financially supported by the dutch asthma foundation and GSK.

Authors’ Affiliations

(1)
Department of Experimental Immunology, Academic Medical Centre, University of Amsterdam, Meibergdreef 9
(2)
Department of Respiratory Medicine, Academic Medical Centre, University of Amsterdam, Meibergdreef 9
(3)
Laboratory of Experimental Intensive Care and Anesthesiology, Academic Medical Centre, University of Amsterdam, Meibergdreef 9

Copyright

© Bal et al; licensee BioMed Central Ltd. 2013

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Advertisement