Figure 3

Eosinophils inhibit the formation of monolayers of the oral cancer cell line, SCC9. A: Representative confluent monolayer of SCC9, 5 days after culture, Nuclei are stained with DAPI for easier identification of SCC9 after washing out eosinophils (red bar = 100 μm). Eosinophils were removed from wells before staining B: Co-culture with eosinophils prevented SCC9 cell growth. ( 25:1 eosinophil-SCC9 ratio in media) C: Mean growth inhibition observed in 5 independent experiments with different eosinophil-SCC9 ratio. IL-5 was used to sustain eosinophil viability except in control group. Reference group (0% growth inhibition) represent SCC9 incubated without addition of cytokines or eosinophils that reached confluence (5 days). SCC9 + IL-5 and SCC9 + IL-5 + IL-2 represent control groups for the effect of cytokines without eosinophil co-incubation. All data are represented by Mean ± SEM * p < 0.05.