Fig. 5
From: Fine particulate matter aggravates smoking induced lung injury via NLRP3/caspase-1 pathway in COPD
NLRP3 is necessary for CSE and PM2.5 induced pyroptosis. (A) NLRP3 expression levels in BEAS-2B cells after treatment with CSE and PM2.5. (B) Transfection with siNLRP3 decreased the protein and mRNA levels of NLRP3. (C) Transfection with siNLRP3 decreased the protein and mRNA levels of activated caspase-1. (D) Inhibition of caspase-1 decreased mRNA levels of IL-1β and IL-18. (E) Silencing of NLRP3 significantly increased cell viability. The data are represented as mean ± SD (n = 2 B and C, n = 3 D, n = 4 E) from three independent experiments. The significance is determined using one-way ANOVA, followed by post hoc Tukey’s test. As for (E), the significance is displayed only between siNC and siNLRP3 for each group. * P < 0.05, ** P < 0.01, *** P < 0.001. NC, negative control