Table 1 Sequence of primers used in qRT-PCR
Gene | Direction | Primer sequence (5’-3’) | Product sizes (bp) |
|---|---|---|---|
IL1β | F | GAGCCTGTCATCTTCGAAACG | 55 |
R | GCACGGGTGCGTCACA | ||
IL6 | F | GCTGAATCTTCCAAAAATGGAGG | 200 |
R | GCTTCAGGATCTGGATCAGTG | ||
TNFα | F | TCCAGAAGTTGCTTGTGCCT | 144 |
R | CAGAGGGCTGTTGATGGAGG | ||
GAPDH | F | CACCCTCAAGATTGTCAGCA | 103 |
R | GGTCATAAGTCCCTCCACGA | ||
β-actin | F | CTGTGCTGTCCCTGTATGCC | 222 |
R | TGTCACGGACGATTTCCCGCT |