Table 1 References of flow cytometry antibodies used for immunophenotyping
From: Tissue-plasminogen activator effects on the phenotype of splenic myeloid cells in acute inflammation
Target antigens | Clones | Fluorochromes | References | Isotypes/clones | Suppliers |
|---|---|---|---|---|---|
CD16/32 | 2.4G2 | - | 553,142 | - | BD |
7-AAD | - | - | 420,404 | - | BioLegend |
CD11b | REA592 | VioBlue | 130–113-810 | human IgG1/REA293 | Miltenyi Biotec |
CD11c | REA754 | APC | 130–110-839 | human IgG1/REA293 | Miltenyi Biotec |
F4/80 | REA126 | PE-Vio770 | 130–118-459 | human IgG1/REA293 | Miltenyi Biotec |
CD80 | REA983 | APC-Vio770 | 130–116-463 | human IgG1/REA293 | Miltenyi Biotec |
CD86 | REA1190 | PE | 130–122-129 | human IgG1/REA293 | Miltenyi Biotec |
MHCII | REA813 | FITC | 130–112-386 | human IgG1/REA293 | Miltenyi Biotec |
CD38 | REA616 | PerCP-Vio700 | 130–109-260 | human IgG1/REA293 | Miltenyi Biotec |
iNOS | REA982 | APC | 130–116-423 | human IgG1/REA293 | Miltenyi Biotec |
CD71 | REA627 | PerCP-Vio700 | 130–109-577 | human IgG1/REA293 | Miltenyi Biotec |
CD206 | C068C2 | APC | 141,708 | Rat IgG2a,k/RTK2758 | BioLegend |
Arg-1 | A1EXF5 | PE | 12–3697-82 | Rat IgG2a,k/eBR2a | Invitrogen |