Fig. 4

cDC phenotype was modulated in an inflammatory setting. A Representative flow cytometry gating strategy used for quantification of total DCs (F4/80⁻ CD11c⁺) and cDCs: cDC1 (F4/80⁻ CD11c⁺ CD11b⁻ MHCII⁺) and cDC2 (F4/80⁻ CD11c⁺ CD11b⁺ MHCII⁺). B Quantification of total DC frequency (Sham WT n = 8; Sham tPA^−/−; LPS WT; LPS tPA^−/− n = 10). C Frequency of cDC1, MFI of MHCII molecules on cDC1 and frequency of CD80⁺ CD86⁺ cDC1 (Sham WT n = 9; Sham tPA^−/−; LPS WT; LPS tPA^−/− n = 10). D Frequency of cDC2 and CD80⁺ CD86⁺ cDC2, MFI of MHCII and CD11b on cDC2 (Sham WT n = 9; Sham tPA^−/−; LPS WT; LPS tPA^−/− n = 10). Data are shown as individual animals with mean ± SD, two-way ANOVA with Bonferroni’s post-hoc