Fig. 3

M1/M2 macrophage polarisation following LPS challenge. A Dot plot analysis of iNOS and CD38 expression for quantification of M1 frequency (F4/80⁺ CD11b⁺ iNOS⁺ CD38⁺), (Sham WT n = 8; Sham tPA^−/−; LPS WT n = 7; LPS tPA^−/− n = 6). B Frequency of MHCII⁺ M1 macrophages and MFI of MHCII molecules on M1 (Sham WT n = 8; Sham tPA^−/−; LPS WT n = 7; LPS tPA^−/− n = 8). C Dot plot analysis of CD206 and Arg-1 expression for quantification of M2 frequency (F4/80⁺ CD11b⁺ CD206⁺ Arg-1⁺) and MFI of CD206⁺ molecules (Sham WT n = 5; Sham tPA^−/−; LPS WT; LPS tPA^−/− n = 7). Data are shown as individual animals with mean ± SD, two-way ANOVA with Bonferroni’s post-hoc