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Fig. 3 | Journal of Inflammation

Fig. 3

From: Lonicera japonica Thunb extract ameliorates lipopolysaccharide-induced acute lung injury associated with luteolin-mediated suppression of NF-κB signaling pathway

Fig. 3

The protective impacts of LTE on the total protein concentration, the ratio of neutrophils in BALF and the expression of inflammatory factors in BALF and tissues of LPS-induced ALI mice. Note: 12 h after LPS challenge, (A) the total protein concentration, (B) the ratio of neutrophils and (C) the contents of IL-1β, IL-6, IL-10 and TNF-α in the supernatant of BALF were collected for analysis and were determined by kits. (D) The mRNA expression levels of IL-1β, IL-6, IL-10 and TNF-α in the tissues of mice were evaluated by qRT-PCR. (E)The protein expression levels of IL-1β, IL-6, IL-10 and TNF-α in the tissues of mice were evaluated by western blot assays. ##p < 0.01 and ###p < 0.001 vs. Sham group; *p < 0.05 and ***p < 0.001 vs. ALI model group

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