Fig. 2

The effects of LTE on the survival rates and lung structure in LPS-induced ALI in mice. Note: (A) Schematic representation of the construction of ALI animal model and LTE/Lut administration. (B) Lung W/D weight ratio was determined in experimental groups. (C) The changes of histological structure of the mouse lung in different groups (20×). HE stainings indicate inflammatory cell infiltration (red arrows), edema (blue arrows), hemorrhage (black arrows), alveolar septal thickening (green arrows) and alveolar epithelial cell shedding (yellow arrows) in sections of the lung. (D) Survival curves in Sham group, ALI and ALI + LTE groups treated with control saline or LTE in mice. Natural death time was recorded by observation every half an hour starting 12 h after LPS. With the use of the Prism software, the death time was shown as a Kaplan-Meier plot. Log-rank test was adopted for comparing survival between groups. Other mice were harvested at 12 h post LPS and lung tissues harvested for analyses of histomorphology and W/D weight ratio were employed to evaluate severity of ALI. Bar = 50 μm. ^##p < 0.01 vs. Sham group; *p < 0.05 vs. ALI model group