Fig. 1From: Colchicine prevents oxidative stress-induced endothelial cell senescence via blocking NF-κB and MAPKs: implications in vascular diseasesColchicine mitigates oxidative stress and oxidative stress-induced senescence in endothelial cells. (A) Immunofluorescence staining for oxidative stress-induced DNA damage marker 8-OHDG, (B) β-gal staining. Quantification of (C) ROS generation, (D) 8-OHDG positive cells and (E) β-gal staining. (F) Western blot showing protein expression of senescence markers. Relative protein expression of (G) KU70, (H) KU80, (I) Lamin B1, (J) P21 and relative mRNA expression of (K) P21. β-actin was used as a loading control. The experiment was performed with biological triplicates. The data was analyzed by applying One-way ANOVA followed by Tukey’s test. Error bars represent SD, (****<0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05), scale bar = 100 μmBack to article page