Fig. 4

HeVc peptide induces ASC speck formation in ASC-cerulean iBMDMs. Murine ASC-cerulean iBMDMs were seeded in ibidi 8 chamber slides at 6 × 10⁴ cells per well 24 h prior to stimulation with 200 µg/ml HeVc peptide for 5 h or Nigericin (6 µM) for 1 h before being fixed with 10% formalin solution and imaged on an FV1200 confocal microscope at 40x objective. Five random z-stacked fields per treatment were taken per condition and the number of cells identified in brightfield and number of ASC specks were quantified using FIJI. Images are representative of 3 independent experiments. Results in (B) are triplicate and are representative of 3 independent experiments and are presented as mean ± SD. ***p < 0.001 Ordinary One-way ANOVA with Dunnett’s test for multiple comparisons