Fig. 3

HeVc peptide-induced IL-1β secretion requires an NLRP3, ASC and Caspase-1 inflammasome complex. Murine iBMDMs deficient in either NLRP3, ASC, or Caspase-1 were primed with 100 ng/ml LPS-B5 for 3 h before being stimulated with A) HeVc peptide (200 µg/ml), B) Silica (125 µg/ml), C) Nigericin (6 µM) or D) poly (dA:dT) for 6 h and supernatants were harvested and assayed for IL-1β secretion by ELISA. Results are pooled and normalized to wildtype response from 3 independent experiments conducted in triplicate and are presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, Ordinary One-way ANOVA with Dunnett’s test for multiple comparisons