Fig. 2

HeVc peptide-induced IL-1β secretion requires phagocytosis, caspase-1 activation and NLRP3 function. Murine iBMDMs were primed with 100 ng/ml LPS-B5 for 2 h before inhibitors of A) phagocytosis (0.5-2 µM Latrunculin A), B-C) caspase-1 activity (1–10 µM Ac-YVAD-cmk, 1–5 µM VX695) and D-E) NLRP3 (1–25 µM MCC950, 87.5-250 µM Probenecid) and were added to cell media for 1 h. Cells were then stimulated with 200 µg/ml of HeVc peptide for 6 h and supernatants were harvested for the detection of IL-1β secretion via ELISA. Results are representative of 3 independent experiments conducted in triplicate and are presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, Ordinary One-way ANOVA with Dunnett’s test for multiple comparisons