Fig. 1

HeVc peptide induces IL-1β secretion in murine immortalized macrophages. (A) Beta aggregation score of C-protein amino acids as derived by Tango software alongside a representative image of where the HeVc peptide is taken from the main C-protein. (B) Murine iBMDMs were seeded at 4 × 10⁴ per well 20 h prior to priming with 100 ng/ml LPS-B5 for 3 h and then challenged with aggregated HeVc peptide (25–200 µg/ml), or NLRP3 stimulants (6 µM Nigericin or 125 µg/ml Silica) as indicated for an additional 6 h. Supernatants were harvested and assayed for IL-1β by ELISA according to manufacturer’s instructions. Results shown are representative of 3 independent experiments conducted in triplicate, presented as mean ± SD where appropriate, *p < 0.05, **p < 0.01, ***p < 0.001. Ordinary One-way ANOVA with Dunnett’s test for multiple comparisons