Fig. 3

Effects of sodium butyrate on the T helper cell differentiation in EAMG mice. Mononuclear cells (MNCs) of the spleens and inguinal lymph nodes were isolated from mice in three groups on day 70. A Th1 cells, (C) Th17 cells, and E Treg cells were detected by flow cytometry. B The percentages of Th1, D Th17, and F Treg cells in MNCs were calculated. Data were from three independent experiments and expressed as mean ± SEM. The significance of differences was assessed by ANOVA, followed by Tukey’s testing as a post-hoc test. (n = 3 mice/group), ns means not significant, *p < 0.05 and **p < 0.01