Fig. 4

Induction of fibrosis in skin and lung tissues in BLM-Induced SSc mice following injection of pcDNA3.1-SARS2-Spike and pLEX307-ACE2-puro (n = 5). (a) Histological analysis of skin and lung tissues isolated from sacrificed SSc mouse models at 5 weeks post-injection, stained with hematoxylin & eosin, Masson’s trichrome, and subjected to immunohistochemistry analysis for α-SMA and collagen type I. (b) Graph representing skin dermal thickness, lung histological score, collagen area, α-SMA, and collagen type I positive area in skin and lung tissues