Fig. 5

sEH deficiency inhibited the CSE-induced secretion of inflammatory cytokines and macrophage migration in BEAS-2B cells. (A-C) The mRNA level of the inflammatory cytokines IL-6, IL-8, and TNF-α in BEAS-2B cells after stimulation with 3% CSE for 0 (CON), 4, 6, 12, and 24 h. (D) Western blot was used to evaluate the protein expression of sEH in each group. (E) Quantification of sEH protein expression by densitometry. (F) qRT-PCR was used to show the changes of sEH mRNA levels in each group. (G-I) After transfection with si-sEH, qRT-PCR was used to assess the mRNA level of IL-6, IL-8, and TNF-α in BEAS-2B cells following stimulation with CSE for 12 h. (J-L) ELISA was used to evaluate the secretion of CSE-induced IL-6, IL-8, and TNF-α in the supernatant of BEAS-2B cells. (M) A Transwell assay was performed to detect the migration ability of THP-1. (N) Statistical result of THP-1 cell migration. (O) Statistical result of cell morphological changes. Data represent the mean ± SD. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001