Fig. 8

Phosphorylation of MAPKs is negatively regulated by cAMP-PKA signal in IgG-IC-treated macrophages. RAW264.7 cells are pre-treated with DMSO or Rolipram (10 µM) for 1 h. Then the cells are treated with or without 100 µg/ml of IgG-IC. RAW264.7 cells are pre-treated with DMSO, Rolipram (10 µM) or Rolipram (10 µM) + H-89 (10 µM) for 1 h. Then the cells are treated with or without 100 µg/ml of IgG-IC. Whole cellular proteins are extracted 30 min later. Activation of p38 MAPK (A and D), ERK1/2 (B and E), and JNK (C and F) is investigated by Western blot assays, respectively