Fig. 2

IgG-IC-induced acute lung injury is attenuated by Roflumilast. Roflumilast is dissolved in DMSO to obtain 20 mg/ml solution. For DMSO groups, mice are treated according to the following principle: 0.05 ml/kg. For Roflumilast groups, mice are treated according to the following principle: 1 mg/kg. Mice are treated by intraperitoneal injection of DMSO + PBS or Roflumilast + PBS, and the total volume is 200 µl. Then mice receive immediate airway deposition of IgG-IC. 4 h later, BAL fluids and whole lung tissues are harvested to analyze pulmonary permeability (A), and MPO activity (B), respectively. Data are expressed as mean ± SEM (N = 6 for control groups, and N = 10 for IgG-IC-treated groups). * and *** suggest p < 0.05 and p < 0.001, respectively