Fig. 5

Effects of TFPI2 overexpression on BMDM polarization under high-glucose stimulation

(a) Western blot analysis of TFPI2, iNOS, Arg-1, and PPARγ in BMDMs (n = 3). (b) Co-immunofluorescence staining of the macrophage marker (CD68, green) and M1 marker (iNOS, red) in BMDMs (n = 3, respectively). (c) Co-immunofluorescence staining of the macrophage marker (CD68, green) and M2 marker (Arg-1, red) in BMDMs (n = 3). (d) Western blot analysis of TFPI2, iNOS, and Arg-1 expression in BMDMs with PPARγ inhibition (n = 3, respectively). NC, normal control; OC, osmotic control; HG, high glucose. TFPI2, transfection with TFPI2 cDNA; vector, transfection with the empty vector. GW9662, PPARγ inhibitor; Data represent mean ± standard deviation (SD). Data were analyzed using one-way ANOVA and Tukey’s post hoc test. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001