Fig. 4

Effects of TFPI2 overexpression on fibroblast activation, migration, and collagen deposition in diabetic MI mice

(a) Western blot analysis of MMP2, MMP9, collagen I, and collagen III in CFs (n = 3). (b, c) Wound healing and Transwell assays for determination of cell migration. (b) Wounds were made through the cell monolayer and imaged using a microscope at 24 h (magnification: ×100). (c) CFs on the external surface of the Transwell chambers were stained using crystal violet and imaged using a microscope (magnification: ×200). (d, e) Western blot analysis and representative staining of TFPI2 to verify TFPI2 overexpression in myocardium samples (n = 3). (f) Representative staining of MMP2 and MMP9 in the infarcted myocardium samples and their quantitative analysis (n = 3). (g) Representative staining of collagen I and collagen III in the infarcted myocardium samples and their quantitative analysis (n = 3). MI, myocardial infarction; DM, diabetes mellitus; TFPI2, transfection with TFPI2 cDNA; vector, transfection with the empty vector. Data represent mean ± standard deviation (SD). Data were analyzed using one-way ANOVA and Tukey’s post hoc test. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001