Fig. 5
From: Inhibition of COX-2 signaling favors E. coli during urinary tract infection
COX-2 inhibition compromised epithelial barrier and increased E. coli infection. Expression of claudin-1 at the (A) mRNA (n = 3) and (B) protein level (n = 3) in 5637 cells, average fluorescence intensity was measured. Intracellular E. coli load in (C) 5637 cells (n = 6), in the (D) urinary bladders of C57BL/6 mice (n = 8) and after treatment with SC-236 (n = 7) and (E) dTHP-1 cells (n = 4). (F) Expression of TLR4 mRNA and (G) TLR4 stained (n = 3) with Alexa-488 (green) and DAPI (blue) for nucleus, average fluorescence intensity of TLR4 was measured in 5637 cells. Data are shown as mean + SEM. Results from mice are presented as median. Significance levels mentioned as *P < 0.05, **P < 0.01, and ***P < 0.001