Fig. 4
From: Inhibition of COX-2 signaling favors E. coli during urinary tract infection
COX-2 inhibition increased antioxidant expression in uroepithelial cells. Representative expression of NRF2 (red), KEAP1 (magenta) and DAPI (blue) before and after 3 h of E. coli infection (n = 3) in 5637 cells. Expression of (B)GCLC and (C)HMOX1 mRNA (n = 3) in SC-236 treated 5637 cells. In vitro analysis was performed in triplicate. 5µM SC-236 was treated for 24 and 36 h for mRNA and protein analysis respectively followed by E. coli infection. Data are shown as mean + SEM. Significance levels mentioned as **P < 0.01 and ***P < 0.001