Fig. 3
From: Inhibition of COX-2 signaling favors E. coli during urinary tract infection
Free radical formation was reduced in COX-2 inhibited uroepithelial cells. Expression of NOS2 mRNA (A) before (n = 4) and (B) after 15 min E. coli infection (n = 3) in 5637 cells. (C) intracellular NOS2 stained (n = 3) with Alexa-594 (red) and DAPI (blue) for nucleus, average fluorescence intensity of NOS2 was measured after 2 h E. coli infection in 5637 cells. (D) Free NO levels (n = 4) and (E) total intracellular ROS (n = 3) in SC-236 treated 5637 cells. (F) For mitochondrial ROS, mitochondria were stained after 1 h E. coli infection (n = 4) and fluorescence intensity was quantified. (G) Released free NO estimation (n = 4) and (H) total intracellular ROS (n = 4) in SC-236 treated dTHP-1 cells. (I) For mitochondrial ROS, mitochondria were stained after 1 h E. coli infection (n = 3) and fluorescence intensity was quantified in dTHP-1 cells were treated with 5µM SC-236 for 24 h followed by E. coli infection. In vitro analysis was performed in triplicate. 5µM SC-236 was treated for 24 and 36 h for mRNA and protein analysis respectively followed by E. coli infection. Data are shown as mean ± SEM. Significance levels mentioned as *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001