Fig. 1

COX-2 inhibition compromised expression of the antimicrobial peptide psoriasin. Expression of (A)PGES2 and (B)S100A7 mRNA before (n = 3) and (C) after 15 min E. coli infection (n = 3) in 5637 cells. (D) Intracellular psoriasin stained (n = 3) with Alexa-488 (green) and DAPI (blue) for nucleus. Average fluorescence intensity of psoriasin was measured after 2 h E. coli infection in 5637 cells. (E) Expression of S100A7 mRNA after 2 h E. coli infection in differentiated THP-1 (dTHP-1) cells (n = 4). (F) Intracellular psoriasin stained (n = 3) with Alexa-488 (green) and DAPI (blue) for nucleus, average fluorescence intensity psoriasin was measured after 30 min E. coli infection in dTHP-1. In vitro analysis was performed either in duplicate or triplicate. Cells were treated with 5µM SC-236 for 24 and 36 h respectively for mRNA and protein analysis followed by E. coli infection. Data are shown as mean ± SEM. Significance levels mentioned as *P < 0.05, **P < 0.01 and ****P < 0.0001