Fig. 2

NaPyr inhibits CSE-induced ferroptosis in A549 cells. (A) Total iron levels in A549. (n = 4;^##P < 0.01 vs. Control;^**p < 0.01 vs. CSE) (B) The fluorescence intensity of MitoSOX in A549 was observed by fluorescence microscope. Scale bar = 100 μm (n = 3;^##P < 0.01 vs. Control;^*P < 0.05,^**p < 0.01 vs. CSE) (C) GSH contents in different experimental groups. (n = 4;^##P < 0.01 vs. Control;^*P < 0.05,^**p < 0.01 vs. CSE) (D) Napyr pretreatment can reduce the generation of ROS induced by CSE. Scale bar = 100 μm (n = 3;^##P < 0.01 vs. Control;^**p < 0.01 vs. CSE) (E) MDA levels in A549 of different experimental groups. (n = 4;^##P < 0.01 vs. Control;^*P < 0.05,^**p < 0.01 vs. CSE) (F) The effect of CSE on MMP in A549 and the pretreatment effect of Napyr. Scale bar = 100 μm (n = 3;^##P < 0.01 vs. Control;^**p < 0.01 vs. CSE)