Table 1 Optimized mass spectrometry settings on a QTrap 5500 mass spectrometer for APAP, AT7519 and isotopically labelled internal standards, operated in positive mode. Voltage (V). All analytes and standards were infused and analysed in positive ion mode
Analyte | Â | Precursor ion (m/z) | Product ion (m/z) | De-clustering potential (V) | Entrance potential (V) | Collision Energy (V) | Collision exit potential (V) |
|---|---|---|---|---|---|---|---|
AT7519 | [M + H]+ | 382.1 | 84.1 | 66 | 9 | 29 | 10 |
d8-AT7519 | [M + H]+ | 390.1 | 89.1 | 66 | 9 | 29 | 10 |
APAP | [M + H]+ | 152.0 | 110.1 | 81 | 9 | 23 | 12 |
d4-APAP | [M + H]+ | 156.0 | 114.0 | 81 | 9 | 23 | 12 |