Fig. 1
From: Identification and validation of biomarkers related to Th1 cell infiltration in neuropathic pain
Flow chart of the analysis step in this study. The datasets were downloaded from the GEO database and the differentially expressed genes (DEGs) that were significantly correlated with NP were screened. Enrichment analysis was performed to describe the function of DEGs. ssGSEA algorithm was used to identify significantly different immune cells. WGCNA package was applied to screen Hub genes related to immune cells. Then, Hub genes were verified through external datasets, and spearman was used to analyze the correlation between Hub genes and immune cells. Next, the expression patterns of these Hub genes were detected at the single-cell level. Finally, the expression level of the Hub gene and its related functions will be verified by in vitro and in vivo experiments