Fig. 5

PKM2 translocates to the nucleus and activates STAT1 through direct interaction. dHL-60s were treated with LPS (1ug/ml) alone for 6 h, pretreated with DASA-58 (20uM) 0.5 h before LPS treatment or treated with DASA-58 alone. A-C Protein levels of PKM2 in the cytoplasm and nucleus of dHL-60s were determined by Western blotting. Hsp90 was used as the loading control for the cytoplasmic protein. Histone H3 was used as the loading control for the nuclear protein. The blot is representative of three independent experiments. D, E Protein level of p-STAT1 was determined by Western blotting. β-actin was used as the loading control. The blot is representative of three independent experiments. F, G Protein–protein interaction between PKM2 and STAT1 was determined by co-immunoprecipitation assay. The blot is representative of three independent experiments. One-way ANOVA was performed. Data are presented as the mean ± SEM. *P < 0.05; **P < 0.01