Table 1 TRY, tryptophan; KYN, kynurenine; KYNA, kynurenic acid; QUIN, quinolinic acid; 5-HT, serotonin; Glu, glutamate; Gln, glutamine, LPS, lipopolysaccharide; HUCPVC, human umbilical cord perivascular cells; HS68, human foreskin fibroblast cells. Data represents mean (± SEM). n = 5–8 mice per group, (except HS68, n = 3). ^aSignificant effect of LPS (versus Control), ^bsignificant effect of HUCPVC (versus LPS), ^csignificant effect of HS68 (versus LPS), Ordinary One-way ANOVA with Tukey’s multiple comparison test. *p < 0.05, **p < 0.005, ***p < 0.0005, ****p < 0.0001

Brain

TRY (nM)

172 (8.09)

209 (7.28)^a*

170 (7.16)^b*

236 (17.1)

KYN (nM)

12.8 (0.96)

61.1 (3.46)^a****

43 (5.97)^b*

47.9 (9.93)

KYN:TRY

0.077 (0.009)

0.338 (0.01)^a****

0.238 (0.025)^b*

0.173 (0.079)^c**

KYNA (nM)

1.257 (0.155)

1.388 (0.060)

1.513 (0.237)

1.39 (0.206)

QUIN (nM)

3044 (604)

7892 (362)^a***

4086 (651)^b**

8090 (1311)

QUIN:KYNA

3081 (511)

5717 (330)^a*

3080 (676)^b*

5790 (229)

5-HT (nM)

385 (40.9)

520 (50.6)

464 (42.6)

507 (3.33)

Glu (µM)

909 (66)

893 (36)

893 (43)

1103 (281)

Gln (µM)

1853 (179.5)

2025 (162.1)

2012 (138.4)

2374 (583)

Plasma

TRY (µM)

0.758 (0.030)

0.756 (0.033)

0.741 (0.046)

0.746 (0.103)

KYN (µM)

0.824 (0.061)

1.155 (0.168)

1.155 (0.133)

0.915 (0.040)

KYN:TRY

1.106 (0.103)

1.71 (0.212)^a*

1.54 (0.104)

1.28 (0.208)

KYNA (µM)

0.159 (0.005)

0.14 (0.014)

0.141 (0.010)

0.151 (0.003)

QUIN (µM)

4.389 (0.392)

4.751 (0.507)

3.808 (0.465)

4.403 (0.336)

QUIN:KYNA

27.9 (3.16)

44.3 (17.7)

30.1 (4.38)

29.2 (2.75)

5-HT (µM)

3.19 (0.411)

3.24 (0.525)

3.12 (0.494)

2.47 (0.070)

Glu (µM)

11.1 (1.82)

12 (2.19)

9.31 (1.88)

10.1 (3.12)

Gln (µM)

170.1 (26.6)

169.5 (24.4)

135.1 (18.1)

176.5 (20.5)