Table 1 Primer sequence list
Gene | Vendor | Sequence |
|---|---|---|
TNF-α | PrimerDesign | AGCCAGGAGGGAGAACAGA (forward) CAGTGAGTGAAAGGGACAGAAC (reverse) |
RIG-I | PrimerDesign | CGATATTTTGAAAGACTTGGGTACA (forward) ATGGCTCCGTTGTTGAGATTG (reverse) |
TLR3 | PrimerDesign | AAGTTATTCGCCCTCCTCTTGA (forward) AGATTCTGGATGCTTGTGTTTGA (reverse) |
IFN-β | PrimerDesign | ATGGAAAGATCAACCTCACCTAC (forward) GGATGGCAAAGGCAGTGTAA (reverse) |
IFN-λ | PrimerDesign | ACCAGGCTCCCAGTGGAA (forward) and TTTTTGAAGGCCTGCAGCTCT (reverse) |
IL-1β | Qiagen | Mm_Il1b_2_SG QuantiTect Primer Assay (QT01048355) |
MDA5 | Qiagen | Mm_Ifih1_1_SG QuantiTect Primer Assay (QT00156338) |
β-actin | Qiagen | Mm_Actb_1_SG QuantiTect Primer Assay (QT00095242) |
CXCL1 | Qiagen | Mm_Cxcl1_1_SG QuantiTect Primer Assay (QT00115647) |
Viperin | Qiagen | Mm_Rsad2_1_SG QuantiTect Primer Assay (QT00109431) |