Fig. 6

Gene expression of IFN-β, IFN-λ, viperin, TNF-α, IL-1β and CXCL1 after i.n. dsRNA administration. dsRNA (100 µg) or saline were i.n. administered to BALB/c, C57Bl/6J or C57Bl/6N mice once per day for three consecutive days and gene expression of IFN-β (A), IFN-λ (B), viperin (C), TNF-α (D), IL-1β (E) and CXCL1 (F) was determined by RT-qPCR in lung homogenates. Data are presented as mean ± SD, as relative to the reference gene β-actin and fold change compared to the mean of saline controls within each strain. Differences between groups were tested using a 2way ANOVA with Tukey’s multiple comparisons test. P-values of < 0.05 are indicted in the graphs, each data point represents one mouse from one experiment (n = 5 per group)