Fig. 5

The effect of i.n. dsRNA administration on protein expression of TLR3, MDA5 and RIG-I. dsRNA (100 µg) or saline were i.n. administered to BALB/c, C57Bl/6J or C57Bl/6N mice once per day for three consecutive days and protein expression of PRRs in lung homogenates was analyzed by western blot. Quantification of immunoblots was performed for TLR3 (A), MDA5 (B) and RIG-I (C). Cropped immunoblots are presented for each of the strains; BALB/c (D), C57Bl/6J (E) and C57Bl/6N (F). Data are presented as mean ± SD, as relative to GAPDH and fold change compared to the mean of saline within each strain. Differences between groups were tested using a 2way ANOVA with Tukey’s multiple comparisons test. P-values of < 0.05 are indicted in the graphs, each data point represents one mouse from one experiment (n = 5 per group)