Fig. 7

Assessment of protein production in the lungs of a murine model of HDM-induced airway inflammation. 8-10-week-old female BALB/C mice were challenged by intranasal administration of 35 µL of whole HDM extract (0.7 mg/mL) in saline for two weeks. BALF and lung tissue lysates obtained from allergen-naïve (n = 5) and HDM-challenged (n = 5) mice were monitored for the abundance of (A) cytokines IL-17A, IL-17A/F, IL-17F and TNF-α, and (B) KC by multiplex Meso Scale Discovery (MSD) platform. Undetectable values of cytokine were assigned a value of 1/4 the minimum detectable limit. (C) BALF of HDM-challenged (n = 4) and allergen-naïve (n = 4) mice were monitored for the abundance of LCN-2 and Elafin by Western blot. (D) Representative blot for LCN-2 and Elafin. Results are shown as boxplots, wherein bars show median and IQR, and whiskers show minimum and maximum points. Each data point represents an individual mouse. Statistical analysis was performed using two-way ANOVA with Bonferroni’s post-hoc test for multiple comparisons. Statistical significance denotes differences compared to control (*p < 0.05, **p < 0.01, ***p < 0.001)