Fig. 4

Comparative analyses of protein abundance profile in HBEC and human PBEC isolated from lungs. (A) HBEC-3KT (n = 5) were stimulated with IL-17A/F (50 ng/mL), TNF-α (20 ng/mL), IFN-γ (30 ng/mL) or cytokine combinations, as indicated for 24 h, and cell lysates were used proteomics profiling by SOMAmer®-based protein array. (B) HBEC-3KT (n = 7) and (C) human PBEC (n = 4 independent donors), were stimulated with IL-17A/F (50 ng/mL), TNF-α (20 ng/mL) or IFN-γ (30 ng/mL), or cytokine combinations, as indicated for 24 h. Tissue culture supernatants were monitored for protein abundance of LCN-2, Elafin, GROα, IL-8 and MMP13, by ELISA after 24 h. Increases in protein abundance was calculated after subtraction of background values in paired unstimulated cells for each biological replicate. Log2 protein abundance values were normalized per row in the heat map to yield a consistent dynamic range for visualization and comparative analyses