Fig. 3

Independent validation of selected protein production in human bronchial epithelial cells. HBEC-3KT were stimulated with either IL-17A/F (50 ng/mL), TNF-α (20 ng/mL) or IFN-γ (30 ng/mL), or cytokines combinations as indicated, for 24 h. Tissue culture supernatants were examined by ELISA for the protein abundance of (A) antimicrobial HDP LCN-2 and Elafin, (B) neutrophil chemokines GROα and IL-8, and (C) MMP-13. Increases in protein abundance are reported after subtraction of background values in paired unstimulated cell samples per replicate. The dashed lines represent average baseline value in unstimulated cells. Results are shown as boxplots, wherein bars show median and IQR, and whiskers show minimum and maximum value. Each data point represents results an independent experimental replicate (n = 7). Fisher’s LSD test for one-way ANOVA was used to determine statistical significance (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001)