Fig. 1

Characterization of the human bronchial epithelial cell proteome. HBEC-3KT were stimulated with IL-17A/F (50 ng/mL) in the presence and absence of TNF-α (20 ng/mL) for 24 h. Cell lysates (14 µg total protein per sample) obtained from five independent experiments were probed using the Slow off-rate Modified Aptamer proteomic array. Increases in log2 protein abundance in response to IL-17A/F, TNF-α, or IL-17A/F + TNF-α was calculated after subtraction of background values in paired unstimulated cells. Pairwise differential analysis was conducted on normalized log2 protein expression values, and Welch’s t-test with a cutoff of p < 0.05 was used to select proteins that were significantly enhanced in response to the combination of IL-17 A/F + TNF-α, compared to either cytokine alone. Log2 protein abundance values were normalized per row in the heat map to yield a consistent dynamic range for visualization. (A) Heat map generated using Multi-Experiment Viewer Version 10.2 to visualize protein expression profile, where each column represents an independent experiment (n = 5 per condition). (B) Volcano plot demonstrating differentially abundant proteins in response to the combination of IL-17A/F and TNF-α, compared to either cytokine alone