Fig. 7
Effects of 5-methoxyflavone on LPS-induced lung inflammation in vivo. (A) Immunofluorescence staining was performed to assess the expression of NOX4, TLR4, NF-ĪŗB and P-P38 in the lung tissues. (B) The fluorescence intensities for NOX4, TLR4, NF-ĪŗB and P-P38 were quantified. (C) Immunofluorescence staining was performed to assess the expression of Nrf2 and HO-1 in the epithelial (EpCAM+) cells of the lung tissues. (D) The fluorescence intensities for Nrf2 and HO-1 in the epithelial (EpCAM+) cells were quantified. (E) Immunofluorescence staining was performed to assess the expression of IL-6 and TNF-Ī± in the epithelial (EpCAM+) cells of the lung tissues. (F) The fluorescence intensities for IL-6 and TNF-Ī± in the epithelial (EpCAM+) cells were quantified. (G) Measurement of pro-inflammatory mediators (IL-6, TNF-Ī± and MCP-1) in the lung homogenates by ELISA assay. (H) Immunofluorescence staining was performed to assess the expression of iNOS in the epithelial (EpCAM+) cells of the lung tissues. (I) The fluorescence intensity for iNOS in the epithelial (EpCAM+) cells was quantified. (J) Immunofluorescence staining was performed to assess the expression of Nrf2 and HO-1 in the macrophage (F4/80+) cells of the lung tissues. (K) The fluorescence intensities for Nrf2 and HO-1 in the macrophage (F4/80+) cells were quantified. (L) Immunofluorescence staining was performed to assess the expression of F4/80, iNOS and P-STAT1 in the lung tissues. (M) The fluorescence intensities for F4/80, iNOS and P-STAT1 in the macrophage (F4/80+) cells were quantified. ##P < 0.01, ###P < 0.001 versus the control group; *P < 0.05, **P < 0.01, ***P < 0.001 versus the LPS group