Fig. 6

Protective effect of 5-methoxyflavone on LPS-induced ALI in mice. (A) Lung pathological changes of LPS-challenged mice with or without 5-methoxyflavone administration (i.g., 25, 50 mg⋅kg^-1⋅d^-1) were examined by H&E staining. (B) The lung injury score of all groups was assessed based on lung pathological changes. (C) Lung/body weight ratios (lung index; an indicator of lung injury) of all groups were measured at 48 h after LPS challenge. (D) Representative images of active-caspase 3 and TUNEL-positive cells in the epithelial (EpCAM⁺) cells of the lung tissues were presented. (E) The fluorescence intensities for active-caspase 3 and TUNEL in the epithelial (EpCAM⁺) cells were quantified. (F) Immunofluorescence staining was performed to assess the expression of Bax and Bcl2 in the epithelial (EpCAM⁺) cells of the lung tissues. (G) The fluorescence intensities for Bax and Bcl2 in the epithelial (EpCAM⁺) cells were quantified. (H) Survival curves for LPS-challenged mice with or without 5-methoxyflavone administration (n = 12 for each group). ^###P < 0.01 versus the control group; ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 versus the LPS group