Fig. 7

Treatment with disulfiram decreased pyroptosis level in LPS-induced ALI and related intestinal mucosa impairment. (A) Levels of pyroptosis relative cytokines IL-18 and IL-1β in serum by ELISA, *P < 0.05, **P < 0.001, ***P < 0.001 by one-way ANOVA followed by post hoc Newman-Keuls Multiple Comparison Test NS means Not significant; Detection of IL-18 and IL-1β in lung tissue of WT mice were quantified by (B) immunofluorescence histochemistry under x200 magnification (scale bar = 100 μm) and (C)expressions of N-GSDMD, IL-18, IL-1β and caspase-1 were explored in lung tissue of WT mice by Wb; (D)The protein levels of N-GSDMD, caspase-1, IL-18 and IL-1β in intestinal mucosa tissues were also detected by Wb