Fig. 6

Disulfiram alleviated ALI-induced intestinal mucosal impairment. (A) H&E staining images of intestinal mucosa tissues from indicated mice under x200 magnification (scale bar = 100 μm); (B) The alveolar-capillary permeability by measuring DAO and D-lactic acid concentration, *P < 0.05, ***P < 0.001 by one-way ANOVA followed by post hoc Newman-Keuls Multiple Comparison Test; NS means Not significant. (C) The protein levels of ZO-1 and Occludin in intestinal mucosa tissues from indicated mice were determined by immunofluorescence

histochemistry, under x200 magnification (scale bar = 100 μm)