Fig. 4

Gsdmd deficiency inhibited pyroptosis level in LPS-induced ALI and related intestinal barrier impairment. (A) Levels of pyroptosis relative inflammatory cytokines IL-18 and IL-1β in serum, **P < 0.01, ***P < 0.001, by one-way ANOVA followed by post hoc Newman-Keuls Multiple Comparison Test; (B)The expression of IL-18 and IL-1β were quantified by immunofluorescence histochemistry and (C) expressions of N-GSDMD, IL-18, IL-1β and caspase-1 were detected by Wb in lung tissue of WT and Gsdmd^−/− groups mice. (D) The expression of N-GSDMD, caspase-1, IL-18 and IL-1β were detected by Wb in intestinal mucosa tissue of WT and Gsdmd^−/− groups mice