Fig. 3

Interrelationships and functional changes among clusters of epithelial cell types expressing ACE2. A The pseudo-time trajectory of epithelial cell clusters 1, 3, 4, 5, and 7. Color from dark to light, indicating the differentiation time from early to late. B Pseudo-time trajectory of cell population differentiation in epithelial cell clusters 1, 3, 4, 5, and 7, which could be used to infer the differentiation relationships among cell populations at the initial step. C Venn diagram showing that the number of differences in cluster 7 vs. 5 was 48 up-regulated genes. D Venn diagram showing 10 down-regulated genes in cluster 7 vs. 5. E KEGG analysis of the Venn diagram intersection of 48 up-regulated genes. F KEGG analysis of the Venn diagram intersection of 10 down-regulated genes. G Gene heat map analysis of clusters 1, 3, 4, 5, and 7. Red indicates higher gene expression. Blue indicates lower gene expression. H GSVA bar chart of cluster 1 vs. cluster 5. I GSVA bar chart of cluster 3 vs. cluster 5. Green indicates no significant difference. Blue indicates significant difference. A T value greater than 0 denotes an up-regulated pathway, while a T value less than 0 denotes a down-regulated pathway