Fig. 1

Serinc2 KO exacerbates CLP-induced ALI. A Bioinformatic analysis showing the regulation of Serinc2 expression after LPS stimulation from indicated GEO databases. B Effect of CLP on Serinc2 expression at the mRNA level in the lung measured by qRT-PCR. **p < 0.01 vs. sham; n = 5. C Immunoblots (left) and Quantification data (right) showing the expression of SERINC2 in the lung tissues of mice with sham or CLP surgeries. GADPH serves as the inner control. *p < 0.05 versus CLP alone. *p < 0.05 vs. sham; n = 5. D Immunohistochemical staining and corresponding statistical results showing the expression of Serinc2 in the lung tissue with or without CLP surgeries. E Schematic showing the strategy to generate Serinc2 knockout (Serinc2^−/−) mouse using CRISPR/Cas9 technology. F Validation of Serinc2 knockout by agarose gel electrophoresis for the PCR products with Serinc2 (Exon2-10) primers. G Immunoblots showing the complete knockout of SERINC2 in the lung tissues. H Representative Evan’s blue images (left) and quantification data (right) showing the impact of Serinc2 knockout on alveolar capillary barrier damage induced by CLP. ***P < 0.001 vs. WT; ###P < 0.001 vs. sham. n = 5. I Representative H&E staining images and the ALI scoring for the H&E staining showing the impact of Serinc2 knockout on CLP-induced pathology. n = 5