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Fig. 1 | Journal of Inflammation

Fig. 1

From: Downregulation of miR-223 promotes HMGB2 expression and induces oxidative stress to activate JNK and promote autophagy in an in vitro model of acute lung injury

Fig. 1

LPS induced oxidative stress and autophagy in alveolar epithelial cells. A MTT assay the effects of different concentrations of LPS on A549 cell viability between 6 and 72 h. B Changes in 8-OHdG expression induced by different concentrations of LPS in A549 cells were detected by ELISA. C Changes in autophagy-related proteins induced by LPS at different concentrations in A549 cells were detected by western blot. D Changes in the apoptotic rate of A549 cells treated with LPS at different concentrations were assessed via flow cytometry. E The fluorescent probe detected the changes in the ROS level in A549 cells induced by different concentrations of LPS. F The concentrations of TNF-α, IL-1β and IL-6 in A549 cells treated with different concentrations were assessed via ELISA. n =3. *P < 0.05, ** P < 0.01, *** P < 0.001

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Journal of Inflammation

ISSN: 1476-9255

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