Fig. 9
From: Pellino1 promoted inflammation in lung injury model of sepsis by TRAF6/ NF-κB signal pathway
The regulation of NF-κB participated in the effects of Pellino1 in lung injury model
SiNF-κB plasmid suppressed NF-κB protein expressions in vitro model following over-expression of Pellino1 (a, c). NF-κB plasmid induced TRAF6 and NF-κB protein expressions in vitro model following down-regulation of Pellino1 (b, c). SiNF-κB plasmid reduced TNF-α, IL-6, IL-1β and IL-18 levels in vitro model following over-expression of Pellino1 (d, e, f, g). NF-κB plasmid promoted TNF-α, IL-6, IL-1β and IL-18 levels in vitro model following down-regulation of Pellino1 (h, i, j, k)
Negative, negative group (n = 3); Pellino1, over-expression of Pellino1 group; siPellino1, down-regulation of Pellino1 group (n = 3); Pellino1 + siNF-κB, over-expression of Pellino1 and down-regulation of NF-κB group (n = 3); siPellino1 + NF-κB, down-regulation of Pellino1 and over-expression of NF-κB group (n = 3). Data are presented as means ± SD using ANOVA followed by Tukey’s post hoc test
##p < 0.01 compared with negative group; **p < 0.01 compared with over-expression of Pellino1 group or down-regulation of Pellino1 group