Fig. 7
From: Pellino1 promoted inflammation in lung injury model of sepsis by TRAF6/ NF-κB signal pathway
The regulation of TRAF6 participated in the effects of Pellino1 in vitro model
SITRAF6 plasmid suppressed TRAF6 and NF-κB protein expressions in vitro model following over-expression of Pellino1 (a, b, e). TRAF6 plasmid induced TRAF6 and NF-κB protein expressions in vitro model following down-regulation of Pellino1 (c, d, e). SITRAF6 plasmid reduced TNF-α, IL-6, IL-1β and IL-18 levels in vitro model following over-expression of Pellino1 (f, g, h, i). TRAF6 plasmid promoted TNF-α, IL-6, IL-1β and IL-18 levels in vitro model following down-regulation of Pellino1 (j, k, l, m)
Negative, negative group (n = 3); Pellino1, over-expression of Pellino1 group; siPellino1, down-regulation of Pellino1 group (n = 3); Pellino1 + siTRAF6, over-expression of Pellino1 and down-regulation of TRAF6 group (n = 3); siPellino1 + TRAF6, down-regulation of Pellino1 and over-expression of TRAF6 group (n = 3). Data are presented as means ± SD using ANOVA followed by Tukey’s post hoc test
##p < 0.01 compared with negative group; **p < 0.01 compared with over-expression of Pellino1 group or down-regulation of Pellino1 group