Fig. 3
From: Dendritic cells transduced with TIPE-2 recombinant adenovirus induces T cells suppression
CD4+ and CD8+ T cell proliferation assay. Transduced DCs were treated with mitomycin C (50 μg/ml) for 20 min at room temperature to act as stimulator cells. Allogeneic responder CD4 + or CD8 + T cells were collected by CD4 or CD8 negative isolation with magnetic beads. CD4+ or CD8+ T cells (2 × 105) were treated with transduced DCs (4 × 105) for 3 days. [3H] Thymidine (0.5 μCi/well) was added 24 h before harvesting using TypeA filtermats and a beta plates cintillation mixture. Disintegrations per minute were determined using a liquid scintillation counter (1205 Betaplate; Perkin-Elmer). Histogram numbers represent the mean ± S.E.M. for each experiment. Three repeated experiments showed similar results